山西大学学报（自然科学版）2005，Vol.28Issue(4)：383-387,5.
过氧化氢酶在DNA修饰的热解石墨电极上的直接电子转移
Direct Electron Transfer of Catalase at a Pyrolytic Graphite Electrode Modified with DNA
摘要
Abstract
The direct voltammetry and electrocatalytic properties of catalase (Cat) in single-stranded or doublestranded (ss-or ds-) calf thymus DNA films cast on pyrolytic graphite (PG) electrodes were investigated. CatDNA film electrodes showed a pair of well-defined and nearly reversible cyclic voltammetry peaks. The formal -0. 209 V with dsDNA and -0. 223 V with ssDNA, respectively. The electron transfer between catalase and PG electrodes was greatly facilitated in the DNA films. At a dsDNA/PG electrode,reduction and oxidation electrontransfer rate constant values of ks, red= 13.5 s-1 and ks ,ox= 13.3 s-1 were obtained. At the ssDNA/PG electrode,the values were ks, red = 21. 0 s-1 and ks, ox = 33. 0 s 1, respectively. UV-Vis absorption spectroscopy demonstrated that catalase retained a near native conformation in DNA films at medium pH. We further investigated the responding with pH,Scanrate and H2O2. Obtaining a series valued information.关键词
过氧化氢酶/DNA/直接电化学/电化学催化Key words
Catalase/DNA/Direct electrochemistry/Electrocatalysis分类
化学化工引用本文复制引用
荣志江,田燕妮..过氧化氢酶在DNA修饰的热解石墨电极上的直接电子转移[J].山西大学学报（自然科学版）,2005,28(4):383-387,5.基金项目
The Overseas Returned Scholar Foundation of Shanxi Province in 2004 （）
