中国林学(英文版)2002，Vol.4Issue(2)：38-42,5.
CaCl2在毛白杨幼苗抗冻性低温诱导中的作用
Role of CaCl2 in Cold Acclimation-Induced Freezing Resistance of Populus tomentosa Cuttings
摘要
Abstract
Populus tomentosa cuttings were treated with 1 mmol@ L- 1 , 5 mmol @ L- 1 , 10 mmol@ L- 1 or 15 mmol@ L-1 of CaCl2 for 1-7 d, respectively, for studying the effects of different concentrations of CaCl2 on freezing resistance. Results indicated that 10 mmol@ L-1 of CaCl2 has greater effect than other concentrations on the enhancement of freezing resistance, and the optimum time of pretreatment was 5 d. In addition, cuttings used for cold acclimation at - 3 ℃ were pretreated with or without 10 mmol@ L- 1 of CaCl2 , 3 mmol@ L 1 of Ca2 + chelator EGTA, 0.05 mmol@ L- 1 of CaM antagonist CPZ or 0.1 mmol@ L- 1 of Ca2+ channel inhibitor LaCl3. The changes in CaM and freezing resistance of all cuttings were investigated. The results showed that cold acclimation at - 3 ℃ increased CaM content and decreased the minimum temperature for 100% survival. The CaCl2 pretreatment enhanced the effect of cold acclimation and obviously increased CaM content and decreased the minimum temperature for 100% survival, but this effect was strongly inhibited by the EGTA, CPZ or LaCl3. It is concluded that the effect of CaCl2 on freezing resistance is associated with its concentration and time of pretreatment, Ca2+-CaM may be involved in the induction of freezing resistance of the cuttings.关键词
毛白杨幼苗,低温锻炼,CaCl2,钙调素,抗冻性Key words
Populus tomentosa cuttings, cold acclimation, CaCl2, CaM, freezing resistance分类
农业科技引用本文复制引用
林善枝,张志毅..CaCl2在毛白杨幼苗抗冻性低温诱导中的作用[J].中国林学(英文版),2002,4(2):38-42,5.基金项目
Supported by the "Tenth-Five-Year Plan" National Key Project in Science and Technology （）
