中华医学杂志（英文版）2005，Vol.118Issue(1)：38-42,5.
Biological effect of velvet antler polypeptides on neural stem cells from embryonic rat brain
Biological effect of velvet antler polypeptides on neural stem cells from embryonic rat brain
LU Lai-jin 1CHEN Lei 1MENG Xiao-ting 2YANG Fan 3ZHANG Zhi-xin 1CHEN Dong2
作者信息
- 1. Department of Hand Surgery, First Hospital of Jilin University, Changchun 130021, China
- 2. Department of Histology and Embryology, Jilin University, Changchun 130021, China
- 3. Department of Pediatric Surgery, First Hospital of Jilin University, Changchun 130021, China
- 折叠
摘要
Abstract
Background Velvet antler polypeptides (VAPs), which are derived from the antler velvets, have been reported to maintain survival and promote growth and differentiation of neural cells and, especially the development of neural tissues. This study was designed to explore the influence of VAPs on neural stem cells in vitro derived from embryonic rat brain. Methods Neural stem cells derived from E12-14 rat brain were isolated, cultured, and expanded for 7 days until neural stem cell aggregations and neurospheres were generated. The neurospheres were cultured under the condition of different concentration of VAPs followed by immunocytochemistry to detect the differentiation of neural stem cells. Results VAPs could remarkablely promote differentiation of neural stem cells and most neural stem cells were induced to differentiate towards the direction of neurons under certain concentration of VAPs.Conclusion Neural stem cells can be successfully induced into neurons by VAPs in vitro, which could provide a basis for regeneration of the nervous system.关键词
neural stem cell/velvet antler polypeptides/differentiation/neuronKey words
neural stem cell/velvet antler polypeptides/differentiation/neuron分类
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LU Lai-jin,CHEN Lei,MENG Xiao-ting,YANG Fan,ZHANG Zhi-xin,CHEN Dong..Biological effect of velvet antler polypeptides on neural stem cells from embryonic rat brain[J].中华医学杂志（英文版）,2005,118(1):38-42,5.
