首页|期刊导航|中国农业科学(英文版)|The Relationship of Color Formation with Related Enzymes and Protein Contents in the Seedcoat of Oilseed Rape (Brassica napus)
中国农业科学(英文版)2004，Vol.3Issue(5)：384-391,8.
The Relationship of Color Formation with Related Enzymes and Protein Contents in the Seedcoat of Oilseed Rape (Brassica napus)
The Relationship of Color Formation with Related Enzymes and Protein Contents in the Seedcoat of Oilseed Rape (Brassica napus)
摘要
Abstract
Three pairs of near-isogenic lines with different genetic backgrounds of yellow-seeded and black-seeded rape (Brassica napus L.) were used as experiment materials to study the relationship of color formation in the seedcoat with enzyme activity and protein content in it. The results showed that with similar genetic backgrounds, phenylalanine ammonia-lyase (PAL) and polyphenol oxidase(PPO) activities in the black-seeded lines were much higher than in their yellow-seeded counterparts and maximum PAL activity in the seedcoat occurred comparatively late while no significant difference was present in glutamine synthetase (GS) between the two types of rape. The plants were treated with red light,blue light, p-hydroxybenzoic acid (a PAL inhibitor), polyvinylpyridoxal (a PPO inhibitor),urea (a protein synthesis promoter) or chloramphenicol (CM, a plastid protein synthesis inhibitor) during seed development. It is speculated that PAL may be primarily responsible for coloration in the yellow seed; PPO may be the main factor contributing to the darkness of the testa of the black genotypes; and nitrogen assimilation is, probably, not directly related to the difference in protein content observed between yellow- and black-seeded genotypes, which may be induced mainly by PAL.关键词
Oilseed rape (Brassica napus)/Seedcoat/Pigment/Protein/PLAKey words
Oilseed rape (Brassica napus)/Seedcoat/Pigment/Protein/PLA引用本文复制引用
LIANG Ying,LI Jia-na..The Relationship of Color Formation with Related Enzymes and Protein Contents in the Seedcoat of Oilseed Rape (Brassica napus)[J].中国农业科学(英文版),2004,3(5):384-391,8.基金项目
The research is part of the 863 Program inthe Ministry of Science and Technology(2002AA207009, 2003AA207131), the Key Program in the National Natural Science Foundation of China (30330400),and the Foundation Program in the Committee of Science and Technol （2002AA207009, 2003AA207131）
