中国医学工程2003，Vol.11Issue(6)：38-44,7.
缺氧/复氧后鼠脑星形胶质细胞基因表达谱的初步分析
Primary analysis of gene expression profiles induced by hypoxia/reoxygenation in cultured cerebral astrocytes
摘要
Abstract
Objective:To study the variety of gene expression profiles of rat cerebral astrocytes after hypoxia/ reoxygenation (H/R) using microarray technology. Methods: Primary cultured cerebral astrocytes (40 days) were exposed to hypoxia (caused by mixed gases composing 95% N2 + 5% CO2)/reoxygenation. These cells were collected and total RNA saamples were gained using TRIzol reagent. cDNA microarray chips comprising 4096 clones were employed to identify the effect of hypoxia/reoxygenation on gene expression profiles in cultured astrocytes. The obtained results were analyzed using Gene Ontology Consortium analysis system, GeneCards database and Pubmed Index system. Results:Gene expression profiles: There were differential expression gene 187 pointsin 4096 measured points, in which known differential expression gene 46 points and unknown differential expression gene 141 points (EST segment). Known differential expression gene: In the 46 points of known differential expression gene, there were down - regulate gene expression 41 points and up- regulate gene expression 5 points; twenty - eight genes related to hypoxia/reoxygenation had not been reported and 18 genes had been reported. Function of known differential expression gene: There were 10 kinds of it. Such as metabolism (23 points), signal transduction (9 points), cell growth(3 points)，structure protein(2 points),immune response(1 point),cell cycle(1 point),cell proliferation(1 point),apoptosis(1 point)and chaperone(1 point).Conclusions:The experiment achieved gene expression profile of cultured rat cerebral astrocytes following exposed to hypoxia/reoxygenation.It was the first discovery of 28 genes related to hypoxia/reoxygenation which had not been reported.The basic rule of differential expression of genes during hypoxia/reoxygenation in cultured rat cerebral astrocytes was discovered pri- marily.关键词
缺氧/复氧/脑星形胶质细胞/基因表达谱Key words
hypoxia/reoxygenation/cerebral astrocytes/gene expression profiles分类
医药卫生引用本文复制引用
邬力祥,王绮如,刘发益,曹莉,赵彦..缺氧/复氧后鼠脑星形胶质细胞基因表达谱的初步分析 [J].中国医学工程,2003,11(6):38-44,7.基金项目
Supported by the Key Project of Chinese Ministry of Education. (No.02174) （No.02174）
