林业科学2008，Vol.44Issue(5)：38-45,插1,9.
离体条件下西黄松成熟合子胚不定芽的诱导及植株再生
Adventitious Bud Induction and Plantlet Regeneration in vitro from Mature Zygotic Embryos of Ponderosa Pine
摘要
Abstract
Adventitious buds were induced from the excised mature zygotic embryos of Ponderosa Pine (Pinus ponderosa). Thehighest induction rate (65.8%) of adventitious buds was obtained with 10-fold multiplication at the highest and 7-foldformation of adventitious buds. Buds proliferation and elongation were achieved on 1/2GD and 1/2SH without growth regulators.A proper amount of activated charcoal promoted adventitious buds elongation and root' growth. The adventitious shoots ofPonderosa Pine were used to induce adventitious roots on 1/2GD and 1/2SH media supplemented with different concentrations ofNAA and GA<,3> . The results showed that NAA played a determinative role in initiating the adventitious roots. Sixteen point sevenpaper, the experimental results showed that roots were successfully induced from cultured mature zygotic embryos of PonderosaPine.关键词
西黄松/成熟胚/离体培养/植株再生Key words
Ponderosa Pine(Pinus ponderosa)/mature zygotic embryos/in vitro/plantlet regeneration分类
农业科技引用本文复制引用
李科友,唐德瑞,李林,朱海兰,赵忠,侯琳..离体条件下西黄松成熟合子胚不定芽的诱导及植株再生[J].林业科学,2008,44(5):38-45,插1,9.基金项目
This research was supported by the State Forestry Administration(98 - 4 - 05) and Key Laboratory of Silviculture on the Loess Plateau of State Forestry Administration(K02-06). （98 - 4 - 05）
