物理化学学报2003，Vol.19Issue(5)：385-388,4.
利用多克隆抗体有效识别中草药中抗表皮生长因子抑制剂
The Novel Application of Polyclonal Antibodies in Recognizing Anti-EGFR Inhibitors Directly from Herb
摘要
Abstract
A polyclonal antibody (PcAb) prepared with piceatannol,a known inhibitor against the epidermal growth factor receptor (EGFR),was adopted as a stationary phase in the chromatographic system.Using the antibody to mimic the enzyme,several anti-EGFR inhibitors were recognized directly from a crude extract of Tibetan herb.Frontal affinity chromatography (FAC) was used here for analyzing molecular interactions between the analytes and an immobilized ligand (in this case the PcAb conjugated to Sepharose CL-4B) by calculating the extent of retardation of the elution front.By combining FAC with mass spectrometry (MS) in the current study,a very efficient and straightforward procedure was developed for analyzing the binding properties of different inhibitors.The novel effective screening of anti-EGFR inhibitors using PcAb from natural resources affords us a new feasible approach for the discovery of lead compounds.关键词
表皮生长因子受体/多克隆抗体/抑制剂/前沿亲和色谱 质谱联用Key words
Epidermal growth factor receptor (EGFR)/Polyclonal antibody (PcAb)/Inhibitor/Frontal affinity chromatography (FAC)-electrospray ionization mass spectrometry(ESI-MS)分类
化学化工引用本文复制引用
朱丽荔,徐筱杰..利用多克隆抗体有效识别中草药中抗表皮生长因子抑制剂[J].物理化学学报,2003,19(5):385-388,4.基金项目
国家自然科学基金( 29992590-2)及北京大学 211资助项目 （ 29992590-2）
