水生生物学报2009，Vol.33Issue(3)：385-390,6.DOI:10.3724/SP.J.0000.2009.30385
洱海螺旋鱼腥藻生长生理特性的初步研究
A STUDY ON THE GROWTH AND SOME PHYSIOLOGICAL CHARACTERISTICS OF ANABAENA SPIROIDES ISOLATED FROM LAKE ERHAI
摘要
Abstract
In recent years, Lake Erhai is experiencing a transition from the mesotrophic to eutrophic condition and cya-nobacterial blooms have a rapid increase in the lake. In this study, the dominant species from the bloom of Lake Erhai, Anabaena spiroides, was isolated and purified. The growth and physiological characteristics of an Anabaena spiroides strain under different environmental conditions were studied to evaluate the influence of environmental factors on Anabaena bloom formation, and the growth was determined by cell biomass and relative growth rate. The results showed that the Anabaena the contents of nitrogen 1.5mmol/L and phosphorus 12μmol/L. The activities of nitrate reductase (NR) and alkaline phophatase (APA), which indicate metabolism of nitrogen and phosphorus, were measured with different concentrations of nitrogen and phosphorus. NR activity of the Anabaena spiroicles strain increased with the raising of nitrogen concentration when nitrogen supply level was low (0-0.36 mmol/L). But the activity of NR was related to the growth under the condi-tion of rich nitrogen supply (0.36-6 mmol/L), i.e. the better strain's growth, the higher NR activity. APA activity of the Anabaena spiroides strain largely depended on the concentration of phosphorus. APA activity was lower with more phos-phorus supply. And Nitrogen did not play an important role in the activity change of APA when phosphorus supply was rich. In addition, it was observed that the Anabaena spiroides strain could grow with low concentration of nitrogen or phos-phorus. This may be related to special regulatory mechanism of nitrogen fixing and phosphorus utilizing of Anabaena.关键词
螺旋鱼腥藻/氮/磷/光照强度/温度/pHKey words
Anabaena spiroides/Nitrogen/Phosphorus/Light intensity/Temperature/pH分类
农业科技引用本文复制引用
常锋毅,潘晓洁,康丽娟,沈银武,李敦海,刘永定..洱海螺旋鱼腥藻生长生理特性的初步研究[J].水生生物学报,2009,33(3):385-390,6.基金项目
湖北省科技攻关项目2006AA305A0402 （）
973项目(2008CB418002) （2008CB418002）
中科院创新课题KZCX1-YW-14-1和KZCX2-YW-426资助 （）
