首页|期刊导航|中国农业科学（英文版）|Isolation and Expression Analysis of a Novel Abiotic Stress-Induced Gene W89 from Wheat
中国农业科学（英文版）2007，Vol.6Issue(4)：391-398,8.
Isolation and Expression Analysis of a Novel Abiotic Stress-Induced Gene W89 from Wheat
Isolation and Expression Analysis of a Novel Abiotic Stress-Induced Gene W89 from Wheat
摘要
Abstract
Water stress and cold stress are important factors restricting plant growth. However, there is little knowledge on the function of stress-responsive genes in plants. Therefore, it is necessary to clone some important genes to study the mechanism of plant adaptation to abiotic stress for improvement of plant resistance. A putative water stress-induced gene, W89, was cloned from the cDNA library of drought-treated wheat seedlings by phage hybridization in situ, and its entire length was obtained using 5'-rapid amplification of cDNA ends (RACE) and reverse transcription-polymerase chain reaction (RT-PCR). The full-length cDNA of W89 consists of 2 392 bp and contains a 1 896 bp open reading frame (ORF) encoding a 631 amino acid protein. Southern blot analysis indicated that W89 was a single-copy gene. RT-PCR analysis revealed that the expression of W89 was upregulated by drought, cold, and abscisic acid (ABA). Amino acid sequence analysis discovered that W89 had a conserved region of DUF248 (pfam03141), which contained a methyltransferase domain with a sterile alpha motif (SAM)-binding motif. Phylogenetic analysis showed that W89 was 66% identical to Oryza sativa dehydration-responsive protein (BAD67956). It was supposed that W89 was a novel dehydration-responsive protein encoding gene. On the basis of the functions of methyltransferase and the SAM-binding motif, the SAM-binding motif of W89 was supposed to be connected with other proteins or transcription factors to transduce stress signals and finally regulate the expression of stress-responsive genes on the early stage of drought stress.关键词
wheat/water stress/cold/gene cloningKey words
wheat/water stress/cold/gene cloning分类
农业科技引用本文复制引用
ZHANG Rui-yue,XU Zhao-shi,LI Lian-cheng,CHEN Ming,MA You-zhi..Isolation and Expression Analysis of a Novel Abiotic Stress-Induced Gene W89 from Wheat[J].中国农业科学（英文版）,2007,6(4):391-398,8.基金项目
This research was financially supported by National 863 Program of China (2006AA10Z1E9 and 2006AA10Z115) and National Special Project for Plant Transgenic and Industry of China (JY03-A-18). （2006AA10Z1E9 and 2006AA10Z115）
