中德临床肿瘤学杂志（英文版）2009，Vol.8Issue(7)：393-396,4.
TLR9在人肺腺癌细胞A549中的表达
Aression of TLR9 in human pulmonary adenocarcinoma cell line A549
Jun Yu 1Tiecheng Pan 1Xiang Wei 1Ligang Liu 1Min Hu 1Fang Yuan 1Jiaduo Li1
作者信息
- 1. Department of Cardiothoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuahn 430030, China
- 折叠
摘要
Abstract
Objective: Being considered as a bridge between the innate immunity and acquired immunity, Toll-like receptors (TRLs) are very important innate immunity moleculars. Recent researchs on the innate immunity have focused on the relation- ship between TLRs and human tumor. This paper investgated the expression and significance of TLR9 in human pulmonary adenocarcinoma cell (A549 cell) and human bronchial epithelial cell (HBE cell). Methods: After culturing A549 cell and HBE cell in vitro, the expression of TLR9 mRNA and protein in both cells were detected by immunocytochemistry, Real-time Quantitative Reverse Transcriptase-Polymerase Chain Reaction (Real-time Quantitative PCR) and Western blot, respectively. Results: By immunocytochemistry staining, TLR9 was mainly expressed in both cells' cell membrane and endochylema as brown-yellow material. It showed that the expressions of TLR9 mRNA and protein in A549 cell were stronger than those in HBE cell (P < 0.01). Conclusion: The results suggest TLR9 might cause the progression of human pulmonary adenocaroinoma, and the mechanism needs to be further investgatied.关键词
TLRg/A549 cell/HBE cell/immunocytochemistry/Real-time Quantitative PCR/western blotKey words
TLRg/A549 cell/HBE cell/immunocytochemistry/Real-time Quantitative PCR/western blot分类
临床医学引用本文复制引用
Jun Yu,Tiecheng Pan,Xiang Wei,Ligang Liu,Min Hu,Fang Yuan,Jiaduo Li..TLR9在人肺腺癌细胞A549中的表达[J].中德临床肿瘤学杂志（英文版）,2009,8(7):393-396,4.
