山西医科大学学报2001，Vol.32Issue(z1)：39-42,4.
抗M2受体的抗肽段抗体对cAMP产生量和钙电流的影响
Effects of anti-peptide antibodies against the second extrace llular loop of human M2 muscarinic receptors on the cAMP production and calci um currents
王文泽 1赵荣瑞 2吴博威 2郭光伟 1唐进 1李家成 1Hjalmarson 3Ake 3Michael 4Fu 4LX4
作者信息
- 1. 山西医 科大学第二临床医学院心胸外科,
- 2. 山西医科大学生理学教研室
- 3. 瑞典哥德堡大学,Sahlgren 医院
- 折叠
摘要
Abstract
Effects of anti-peptide antibodies aga inst the second extracellular loop of human M2 muscarinic receptors on the cAMP production and calcium currents(ICa) in guinea pig ventricular m yocytes we re studied by using radioimmunoassay and whole-cell patch clamp technique an d a comparison was also made with those of the muscarinic receptor agonist. Both muscarinic receptor agonist carbachol (Carb 10 μmol/L) and anti-pepti de antibodies (Abs 100 nmol/L) could decrease the isoprenaline (Iso 0.8 μm ol/L) -induced increases of cAMP〔from (108.2±7.0) to (88.4±7.2) pmol/(mg* min)for Carb and (88.6±5.1) pmol/(mg*min) for Abs respectively〕 production and i ncreases of ICa〔from (53.5± 7.0)% to (13.0±2.0)% for Carb and (66.9± 10.5)% to (34.5±8.0)% for Abs res pectively〕. The muscarinic receptor antagonist atropine (Atr) was able to prevent these effects of Carb and Abs.These results indicated that the anti-p eptide antibodies against an epitope located in the second extracellular loop o f human M2 muscarinic receptors, similar to muscarinic receptor agonist , could decrease the β-receptor agonist stimulated incre ase of ICa by decreasing the β -receptor agonist stimulated increa se of cAMP productions and therefore could have an effect on their target receptor. These results further suggest that autoimmunity may participate in the pathogenesis of human idiopathic dilated cardiomyopathy(DCM) and the seco nd extracellular loop of human M2 musarinic receptor could be the ma in immunodominant region.关键词
抗体,抗肽段/受体,毒蕈碱/腺苷环一磷酸分类
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王文泽,赵荣瑞,吴博威,郭光伟,唐进,李家成,Hjalmarson,Ake,Michael,Fu,LX..抗M2受体的抗肽段抗体对cAMP产生量和钙电流的影响[J].山西医科大学学报,2001,32(z1):39-42,4.
