中华医学杂志（英文版）2001，Vol.114Issue(1)：39-43,5.
脂肪组织源性肿瘤与抑癌基因p53关系的研究
Relationship between tumor suppressor gene p53 and tumors of adipose tissue
王娅兰 1丘钜世 2熊敏2
作者信息
- 1. 重庆医科大学病理教研室重庆 400016
- 2. 中山医科大学病理教研室广州 510089
- 折叠
摘要
Abstract
Objective To investigate the relationship between p53 gene and tumors of adipose tissue at the level of protein and gene.
Methods Immunohistochemical LSAB, PCR-SSCP and DNA sequencing were used in 82 cases.
Results p53 protein is expressed only in liposarcomas, in which the positive staining rate was 48.08% (25/52). In different subtypes of liposarcomas, the positive staining rate in well differentiated liposarcomas was 30.00% (9/30), which is much lower than that of the poorly differentiated liposarcomas (P<0.005). Abnormality in the single-stranded DNA pattern was determined in 2 samples (pleomophic liposarcomas) by PCR-SSCP analysis. Missense mutations in exon 8 codon 268 of p53 gene (AAC→ATC) were detected by DNA sequencing. Another heterozygotic cosense mutation may exist at exon 6 codon 221 of p53 gene (GAG→GAA).
Conclusions The data suggest that the p53 protein has a relationship with development, differentiation and malignancy of liposarcoma. Detecting the level of p53 protein expression may be valuable in evaluating the level of differentiation and malignancy of liposarcoma. There appear point mutation on exon 8,6 of p53 gene.关键词
脂肪组织源性肿瘤 p53 免疫组化 PCR-SSCP DNA序列分析Key words
adipose tissue tumor/p53 gene/immunohistochemistry/PCR-SSCP/DNA sequencing分类
医药卫生引用本文复制引用
王娅兰,丘钜世,熊敏..脂肪组织源性肿瘤与抑癌基因p53关系的研究[J].中华医学杂志（英文版）,2001,114(1):39-43,5.
