生态与农村环境学报2009，Vol.25Issue(3)：39-43,48,6.
黄土高原土壤中细菌群落结构多样性的PCR-DGGE分析
Diversity of Bacterial Community Structure in Soils of Loess Plateau
摘要
Abstract
Soil samples were collected from five regions and 26 soil samples from the Xifeng Profile of the Loess Plateau.Total DNA was extracted from those samples with the aid of bacterial universal primer and amplified by means of PCR and its product analyzed with the DGGE technique to explore diversity of bacterial communities.Oriented towards the features of the microbes in the soil of the plateau,conditions of the DGGE experiment were optimized with an optimal denaturant gradient ranging between 40% and 70%,and an optimal electrophoresis duration being 17 hours (100 V).Analysis of DGGE profiles of bacteria in soils at various depths revealed that there are two different types of bacterial community structures in soils at various depths in the Loess Plateau,which is presumably attributed to changes of the warm-humid environment caused by monsoon climatic conditions,and ice age.In the age when monsoon got stronger in summers and became weak in winters,the climate was warm and humid,and weathering and soil formation were intensive,which showed multiple and dense bands in the DGGE profile,whereas conversely,weathering and soil formation are weak,which showed sparse bands in the profile.The determination of Shannon-Weiner indexes of soil bacteria in the five regions of the plateau indicated that the Shannon-Weiner indexes were influenced not only by the amount of bands,but also by brightness and moving rates of the bands关键词
黄土高原/微生物多样性/群落结构/PCR-DGGEKey words
Loess Plateau/bacterial diversity/community structure/PCR-DGGE分类
生物科学引用本文复制引用
潘雪莲,黄晟,方昊,徐军,郭晓峰,陈旸 ,崔益斌..黄土高原土壤中细菌群落结构多样性的PCR-DGGE分析[J].生态与农村环境学报,2009,25(3):39-43,48,6.基金项目
国家自然科学基金重点项目(40331001) （40331001）
