中国组织工程研究与临床康复2009，Vol.13Issue(20)：3981-3985,5.DOI:10.3969/j.issn.1673-8225.2009.20.038
成骨生长肽对骨髓基质细胞增殖和向成骨方向分化的影响
Effect of osteogenic growth peptide on proliferation and differentiation of bone marrow stromal cells
黄姣 1徐凌 2王璐2
作者信息
- 1. 重庆医科大学附属口腔医院牙周科,重庆市,400015
- 2. 重庆医科大学附属口腔医院修复科,重庆市,400015
- 折叠
摘要
Abstract
BACKGROUND: Osteogenic growth peptide (OGP) is a newly discovered growth factor to promote osteogenesis, the effect on proliferation and differentiation of bone marrow stromal cells (MSCs) arouses more and more attention.OBJECTIVE: To elucidate the effect of OGP on proliferation and differentiation of MSCs in vitro.DESIGN, TIME AND SETTING: A randomized COntrol experiment was performed at the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education from February to June 2006.MATERIALS: A 6-month-old male Sprague-Dawley rat was used for the extraction of MSCs; and OGP was provided by Sigma Company, USA.METHODS: MSCs were cultured individually in a-MEM medium containing various COncentrations of OGP (10-11 mol/L,10-10 mol/L, 10-9 mol/L, 10-8 mol/L and 10-7 mol/L) and fetal bovine serum. The cells in the control group were cultured with α-MEM medium containing FBS alone.MAIN OUTCOME MEASURES: Identification of MSCs; cell proliferation using MTT method; alkaline phosphatase (ALP) activity using enzyme-linked immunosorbent assay; core binding factorα1 (Cbfa1) mRNA expression using RT-PCR.RESULTS: The addition of OGP resulted in an increase of cell proliferation (10-9mol/L OGP, P < 0.05), ALP activity (10-8 mol/LOGP, P < 0.05) and Cbfal mRNA expression (P < 0.05).CONCLUSION: OGP stimulates proliferation and sequential differentiation of MSCs via inducing Cbfal mRNA expression.关键词
成骨生长肽/骨髓基质细胞/细胞增殖/碱性磷酸酶/核心结合因子1分类
医药卫生引用本文复制引用
黄姣,徐凌,王璐..成骨生长肽对骨髓基质细胞增殖和向成骨方向分化的影响[J].中国组织工程研究与临床康复,2009,13(20):3981-3985,5.
