现代妇产科进展2003，Vol.12Issue(5)：398-400,3.
The in vivo study of myeloprotection by GST-π gene transfected human cord blood hematopoietic stem cells transplantation
The in vivo study of myeloprotection by GST-π gene transfected human cord blood hematopoietic stem cells transplantation
Yang Xingsheng 1Yu Chenghao 2Kong Yawei 3Jiang Jie 1Dong Ruiying 1Cui Baoxia 1Wang Lijie 1Jiang Sen1
作者信息
- 1. Department of Obsterics and Gynecology
- 2. Department of Pharmacy,Qilu Hospital,Shandong University,Jinan 250012
- 3. School of Life Science,Shandong University
- 折叠
摘要
Abstract
Objective:To investigate the influence of GST-π gene transfer into human cord blood hematopoietic stem cells on their drug resistance against anti-tumor drugs in vivo.Methods:GST-π gene transfection into human cord blood CD34+ cells was carried out using a retrovirus vector PLJ-GST-π with the aid of fibronectin.Successful gene transfer was confirmed by in vitro colony assay and RT-PCR.GST-π gene transduced human cord blood CD34+ cells were then engrafted into 4-week-old total body irradiated NOD/Scid mice and carboplatin was intraperitoneally administered sequentially at 4 weeks interval 4 weeks after engraftment.Results:Peripheral blood(PB) WBC was significantly higher in GST-π mice than control mice after 2 course of carboplatin.Retroviral GST-π expression in bone marrow hematopoietic progenitor cells of recipient mice was detected by RT-PCR 16 weeks after Xenotransplantation.Conclusion:The transfection of GST-π gene could confer,to some extent,resistance to cord blood stem cells against carboplatin in vivo. 关键词
Fetal blood/Hematopoietic stem cells/Genes,GST-π/Drug resistanceKey words
Fetal blood/Hematopoietic stem cells/Genes,GST-π/Drug resistance分类
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Yang Xingsheng,Yu Chenghao,Kong Yawei,Jiang Jie,Dong Ruiying,Cui Baoxia,Wang Lijie,Jiang Sen..The in vivo study of myeloprotection by GST-π gene transfected human cord blood hematopoietic stem cells transplantation[J].现代妇产科进展,2003,12(5):398-400,3.
