山西大学学报（自然科学版）2007，Vol.30Issue(3)：400-405,6.
甜荞愈伤组织分化研究
Study on Callus Differentiation of Common Buckwheat*
摘要
Abstract
Calli from cotyledon and hypocotyl explants of four cultivars of common buckwheat were induced on Murashige and Skoog (MS) basal medium and the effects of plant growth regulators and sucrose concentrations on callus differentiation were investigated.The results showed that the combination of 17.8 μmol/L～26.6 μmol/L(4.0 mg/L～6.0 mg/L) of 6-benzyladenine (BA) with 0.9 μmol/L(0.2 mg/L) of 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing differentiation and generation of adventitious buds.Comparative studies using different sucrose concentrations (1.5 %～6.0 %) demonstrated that higher sucrose concentrations (4.5 %～6.0 %) promoted root differentiation and enhanced callus growth.Addition of activated charcoal into the culture media inhibited root differentiation.关键词
荞麦/愈伤组织分化/2,4-D/BA/活性炭/蔗糖Key words
buckwheat/callus differentiation/2,4-dichlorophenoxyacetic acid/6-benzyaldenine/activated charcoal/sucrose分类
农业科技引用本文复制引用
郝建平..甜荞愈伤组织分化研究[J].山西大学学报（自然科学版）,2007,30(3):400-405,6.基金项目
Shanxi Natural Science Foundation(19961020) （19961020）
