首页|期刊导航|中国农业科学(英文版)|Effect of the Flanking Sequence Architecture of Translation Initiation AUG Codon on Gene Expression Level in Rice
中国农业科学(英文版)2004，Vol.3Issue(6)：401-408,8.
Effect of the Flanking Sequence Architecture of Translation Initiation AUG Codon on Gene Expression Level in Rice
Effect of the Flanking Sequence Architecture of Translation Initiation AUG Codon on Gene Expression Level in Rice
摘要
Abstract
The relationship between the codon usage bias, gene expression level and the AUG context (from -20 to +6 positions relative to the initiator AUG codon) was examined in 541unigene sequences of rice. A significant correlation for CAI values (codon adaptation index) was observed at five nucleotide positions (-19, -18, -9, -4, +5), eight (-19, -18,-14, -9, -6, -4, -1, +5) for CPP (codon preference parameter), and seven (-18, -16, -15,-9, -7, -1, + 6) for mRNA abundance in the flanking sequence of the initiator AUG codon respectively, but a significantly positive correlation for both CAI and CPP at two positions (-4 and +5), indicating that both those positions are evolutionally under the natural selection constraint at the translational level. By site-directed mutagenesis at seven specific positions (-18, -16, -15, -9, -7, -1 and + 6) for allergenic protein that had the highest mRNA abundance in this study, its expression level decreased dramatically 63.3 and 72.5% respectively, indicating the importance of those 7 positions for gene expression. A highly positive correlation (r= 0.625, P＜ 0.01) between AUGCAI and GC content in the flanking sequence of the initiator AUG codon showed a more effective higher GC content on translation initiation efficiency. The strong preference for G or C at those 8 positions (-6, -5, -3, -2, -1, +4, +5 and +6) in the AUG context suggested that an important factor in modulation of the translation efficiency, as well as synonymous codon usage bias, particularly in highly expressed genes.关键词
Rice/AUG context/Codon usage bias/Gene expression level/Simulation of pointmutationKey words
Rice/AUG context/Codon usage bias/Gene expression level/Simulation of pointmutation引用本文复制引用
LIU Qing-po,TAN Jun,XUE Qing-zhong..Effect of the Flanking Sequence Architecture of Translation Initiation AUG Codon on Gene Expression Level in Rice[J].中国农业科学(英文版),2004,3(6):401-408,8.基金项目
This work was supported by the National Natural Science Foundation of China (39870421) and the Key Research Project of Zhejiang Province, China (2003C22007). （39870421）
