植物研究2006，Vol.26Issue(4)：405-410,6.
荧光定量PCR检测干旱胁迫下长春花Crlea基因的相对表达
Comparative expression analysis of Crlea gene in Catharanthus roseus under drought stress by real-time quantitative PCR
摘要
Abstract
A full-length Crlea(Crlea for Catharanthus roseus late embryogenesis abundant) gene was first isolated from Catharanthus roseus. Gene expression profiles of Crlea gene in leaves and roots under drought stress were monitored by real-time quantitative PCR. The results showed that a similar accumulation pattern of Crlea gene in leaves and roots over the observation period of 0.5 to 8 hours. The expression of Crlea mRNA was strengthened with the prolongation of stress time. In leaves, expression amounts of Crlea gene were 9.984 and 20.431 times higher than that of control respectively at 6 and 8 h. Similarly, the expression amounts of Crlea gene in root obviously increased (2.831 times higher than that of control) at 8 h. Primary results show the expression of Crlea gene is non-tissue-specific and up-regulated under drought stress.关键词
长春花/干旱胁迫/晚期胚胎丰富蛋白/荧光定量/相对表达Key words
Catharanthus roseus/drought stress/lea gene/real time quantitative PCR/comparative expression分类
农业科技引用本文复制引用
祖元刚,聂明珠,房思良,于景华,郭晓瑞..荧光定量PCR检测干旱胁迫下长春花Crlea基因的相对表达[J].植物研究,2006,26(4):405-410,6.基金项目
Supported by the State Key Basic Research and Development Plan of China (G19990160) （G19990160）
