分析化学2001，Vol.29Issue(4)：406-409,4.
高效液相色谱手性流动相法拆分甲状腺素对映体
Resolution of Racemic Thyroxine with Chiral Additive asMobile Phase by High Performance Liquid Chromatography
王荣 1谢景文 2贾正平 3胡晓丽 4陈立仁1
作者信息
- 1. 中国科学院兰州化学物理研究所,
- 2. 兰州军区兰州总医院药品器材科,
- 3. 兰州军区兰州总医院药品器材科，兰州 730050
- 4. 兰州军区医学高等专科学校教务部,
- 折叠
摘要
Abstract
A high performance liquid chromatographic (HPLC) method using triethylamine (TEA), copper *" acetate and L-proline as the chiral mobile phase (CMP) and the enantiomric analysis of thyroxine (T4) are described. The influence of the mobile phase parameters including the TEA concentration, manipulation pH, concentration of Cu*", concentration of L-proline, temperature of column and flow rate of eluent on the enantiomers separation of thyroxine was studied. The HPLC-CMP is a simple method for separation and determination of D，L-T4 in drug or in human plasma. It can be used to therapeutic drug or adverse drug reactions monitor.关键词
甲状腺素/对映体/高效液相色谱手性流动相法分类
化学化工引用本文复制引用
王荣,谢景文,贾正平,胡晓丽,陈立仁..高效液相色谱手性流动相法拆分甲状腺素对映体[J].分析化学,2001,29(4):406-409,4.
