作物学报2000，Vol.26Issue(4)：406-410,5.
拟南芥菜花药绒毡层启动子的克隆和序列分析
Isolation and Sequencing Analysis of the Promoter for an Anther Specific Gene from Arabidopsis thaliana
摘要
Abstract
The upstream regulatory region of the tapetal-specific gene A9 was amplified from Arabidopsis thaliana genome by polymerase chain reaction and cloned into HincⅡ and Kpn Ⅰ site of pUCl8．Sequence analysis showed that the cloned fagment contained 364 nucleotides，and shared a sequence homology of 99．2％with the reported A9 promoter.The putative TATA box was present at posidon —76 to —69．Two motifs，TGTGG and TGTGA，which were considered to be responsible for the specificity and enhancement of gene expression in the tapetal，were fOund within—291 to—287 and—243 to—239 regionrectively．Gene construct thatcontained the β-glucuronidase(GUS)gene under the control of A9 promoter or CaMV35s promoter was introduced into anthers and calli of maize by particle bombardmend．The GUS activity was detected only in anthers by fluorometric assay．关键词
拟南芥菜/启动子/花药特异表达/遗传转化Key words
Arabidopsis thaliana/Promoter/Anther-specific expression/Genetic transformation分类
农业科技引用本文复制引用
刘大文,谢友菊,王守才,戴景瑞..拟南芥菜花药绒毡层启动子的克隆和序列分析[J].作物学报,2000,26(4):406-410,5.基金项目
本研究得到国家“九五”攻关项目支持(96-002-02-04) （96-002-02-04）
