激光生物学报2008，Vol.17Issue(3)：406-411,6.
低能离子诱变选育华根霉高产L-乳酸菌株的研究
Screening High-yield Bacteria Rhizopus chinensis Carrying L-lactic Acid by Low Energy Ions Implantation
周小楠 1叶辉 2朱苏文 1程备久1
作者信息
- 1. 安徽农业大学生命科学学院,安徽,合肥,230036
- 2. 皖南医学院微生物学与免疫学教研室,安徽,芜湖,241000
- 折叠
摘要
Abstract
The L-lactic acid producing condition of Rhizopus chinensis was optimized. The high-yield mutant strain was obtained by the method of implanting nitrogen ion ( N+ ) into Rhizopus chinensis in 10 keY. In order to compare differences at the molecular level between the original strain and the mutant strain, 108 pieces of random primers were designed to clarify the genetic background by randomly amplified polymorphie DNA (RAPD). The results showed that the final concentration of L-lactic aeid reached 28.8 g/L when the optimal fermentation medium consisted of glucose 90 ‰, (NH4)2SO42‰, KH2PO4 0. 6‰, MgSO4 ·7H2O 0.25 ‰, ZnSO4·7H2O 0.05 ‰, CaCO3 50.0 ‰ and 5 % volume of seeds and fostered at 34 ℃. A high-yield mutant strain was obtained using N+ implantation with the dose of 1.30×1015 ions/cm2, whose L-lactic acid yield was 17 % higher than that of the original one, which had high genetic stability. By RAPD analysis, 3 primers whose amplification products showed polymorphic eharacter were selected from 108 primers, which can be used to discern the mutant strain.关键词
离子注入/华根霉/L-乳酸/RAPDKey words
ion implantation/ Rhizopus chinensis/ breeding/ L-lactic.acid/ RAPD分类
生物科学引用本文复制引用
周小楠,叶辉,朱苏文,程备久..低能离子诱变选育华根霉高产L-乳酸菌株的研究[J].激光生物学报,2008,17(3):406-411,6.
