中国临床康复2003，Vol.7Issue(30)：4066-4067,2.
川芎嗪对花生四烯酸诱导血管内皮细胞凋亡的保护作用
Protective effect of tetramethylpyrazine on apoptosis of vascular endothelial cells induced by arachidonic acid
摘要
Abstract
AIM:To study the protective effects and mechanism of Ligustrazine against damage and apoptosis in human umbilical vascular endothelial cells(HUVECs) induced by 160 μ mol/L arachidonic acid(AA). METHODS:The survival rate of cells was detected by MTT,the release rate of lactate dehydrogenase(LDH) and malondialdehyde(MDA)content in endothelial cells(EC) are measured by colorimetric assay.Hoechst 33258 fluorescence staining was used to observe morphological changes of nucleus and determine apoptotic ratio.Agarose gel electrophoresis was used to detect DNA fragmentation. RESULTS:MP(0.25- 1.0 mmol/L) prevented the inhibitory effect of viability induced by AA(t=2.52- 3.14,P< 0.05).TMP(0.25- 1.0 mmol/L) partly prevented the increase in the release rate of LDH and MDA contents induced by AA(t=3.27- 5.88,t=4.64- 7.41,P< 0.05).Cells treated with 160 μ mol/L AA showed typical morphological changes of apoptosis.A " DNA ladder" was also observed.TMP(0.25- 1.0 mmol/L) partly decreased the ratio of apoptotic cells. CONCLUSION:TMP can protect AA induced apoptosis in EC and the mechanism of its action may be related to anti lipid peroxidation.关键词
花生四烯酸/细胞凋亡/脂质过氧化作用分类
医药卫生引用本文复制引用
张冀,王韵,汪炳华,陈丽达,夏腊菊..川芎嗪对花生四烯酸诱导血管内皮细胞凋亡的保护作用[J].中国临床康复,2003,7(30):4066-4067,2.基金项目
Supported by:Hubei Education Commission, No.99A060 （）
