中国临床康复2004，Vol.8Issue(20)：4110-4112,3.
脊髓损伤中碱性成纤维细胞生长因子的变化与意义
Changes and significance of basic fibroblast growth factor expression after experimental spinal cord injury
杨星光 1眭述平 1于晓雯 1王韬 1王建伟 1张长青1
作者信息
- 1. 上海交通大学附属第六人民医院骨科,上海市创伤骨科临床医学中心,上海市,200233
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摘要
Abstract
BACKGROUND: Basic fibroblast growth factor(bFGF) is known to be neurotrophic in vitro. However, the changes and effect of it in vivo after spinal cord injury(SCI) are still the subject of controversy.OBJECTIVE: To explore the self-repair mechanism following SCI and find the available methods for effective treatment.DESIGN: A randomized controlled trial was conducted.SETTING and PARTICIPANTS: The experiment was completed in Central Laboratory of Zhongshan Hospital, Fudan University with 55 SD rats, weighing 200 - 230 g that were provided by Shanghai Laboratory Animal Center of Chinese Academy of Sciences.INTERVENTIONS: The rats were randomly divided into sham group(7rats) and 1st, 4th, 7th, 14th, 21st and 28th day injury groups(8 rats in each group). T8 SCI model was made with improved Allen's method.MAIN OUTCOME MEASURES: The distribution and change of bFGF and GFAP protein in T8 and adjacent segments were detected in time course. And the histological observations and image analysis were performed for all sections.RESULTS: The bFGF positive cells increased obviously in T8 and adjacent segments. Image analysis showed that mean bFGF absorbance in T8 segment on the 4th, 7th, 14th and 21st day after injury was significantly different from that of control group respectively( P ＜ 0.05) with the highest positive amount on the 14th day. GFAP immunoreactivity revealed that after injury,there were large number of reactive astrocytes in grey substance and they account for most of the bFGF positive cells.CONCLUSION: Injury to spinal cord induces temporal increased expression of endogenous bFGF, which suggests that FGF may play a part in neuron nutrition and self-protection.关键词
骨髓损伤/成纤维细胞生长因子,碱性/大鼠分类
医药卫生引用本文复制引用
杨星光,眭述平,于晓雯,王韬,王建伟,张长青..脊髓损伤中碱性成纤维细胞生长因子的变化与意义[J].中国临床康复,2004,8(20):4110-4112,3.
