青岛海洋大学学报(自然科学版)2001，Vol.31Issue(3)：411-414,4.
中国对虾淋巴组织的长期原代培养和化学诱变初探
The Long-Term Primary Culture of Penaeus Chinensis Lymphoid Tissue and a Preliminary Study of its Chemical Transformation
郎刚华 1王勇 2汪岷 1陈西广1
作者信息
- 1. 青岛海洋大学海洋生命学院,
- 2. 中国科学院海洋研究所,
- 折叠
摘要
Abstract
Some factors affecting the primary culture of Penaeus chinensis lymphatic cells were studied. It was found that simply rinsing the shrimp in 75% ethanol could obtain satisfactory result in sterilization, the optimal inoculating density was 240～260 blocks/25cm2. After cultured for about 30 days, the cells stopped growing due to contact inhibition. However, after the inhibition was broken by scratching away some cells, the cells continued to grow. Moreover, after we had tried to mutagenize the cells with N-methyl-N｀-nitrosoguanidine (MNNG), some transformed cells had some morphological changes and grew in multilayer. In the meantime, many foci turned up.关键词
中国对虾/淋巴组织/组织培养/原代培养/化学诱变/N-甲基-N′-亚硝基胍（MNNG）分类
生物科学引用本文复制引用
郎刚华,王勇,汪岷,陈西广..中国对虾淋巴组织的长期原代培养和化学诱变初探[J].青岛海洋大学学报(自然科学版),2001,31(3):411-414,4.
