物理化学学报2001，Vol.17Issue(5)：412-415,4.
低能离子与腺苷酸组分水溶液作用合成腺苷酸
Synthesis of Adenosine 5′ monophasphate from Its Constitutes by Implanting Low Energy N＋ into Aqueous Solution
石怀彬 1邵春林 1王相勤 1余增亮1
作者信息
- 1. 中国科学院等离子体物理研究所离子束生物工程中心 ,
- 折叠
摘要
Abstract
Low energy ion implantation has been widely used for mutation breeding,DNA delivery and microbe improvement et al.since 1980′ s,but for a long time such kind of implantation was performed only to dry samples because it needed a vacuum chamber,for this reason,a new apparatus (see Fig.1)was designed in this paper to induce chemical reactions by introducing low energy ions into aqueous solution.In order to explore the origin of life induced by low energy ions and prebiotic synthesis of nucleotide via reaction between low energy ions and aqueous solution under primitive earth conditions,low energy N＋ was implanted into aqueous solution containing adenine,D ribose,and NH4H2PO4.HPLC analysis showed that a new substance was synthesized which was eluted as 5′ AMP (to see Fig.2),then it was separated from the sample and analyzed by 1H NMR spectrum.It was confirmed that 5′ AMP was formed in the experiment by 1H NMR spectrum (see Fig.3).The relation between yield of 5′ AMP and irradiated time was obtained (see Fig.4).Mechanism of the production of 5′ AMP was also discussed.The result is interesting for the formation and accumulation of nucleotide in the primitive oceans on the early earth.关键词
低能离子/腺苷酸/前生物合成/高效液相色谱(HPLC)分类
生物科学引用本文复制引用
石怀彬,邵春林,王相勤,余增亮..低能离子与腺苷酸组分水溶液作用合成腺苷酸[J].物理化学学报,2001,17(5):412-415,4.
