首页|期刊导航|中国农业科学（英文版）|Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers
中国农业科学（英文版）2006，Vol.5Issue(6)：412-416,5.
Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers
Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers
摘要
Abstract
S-a, S-b and S-c are three loci for F1 pollen sterility in cultivated rice (Oryza sativa L.). Taichung 65 (T65) is all Sj/Sj at these three loci, while its F1 pollen sterile near-isogenic lines, TISL2 (S-b), TISL4 (S-a) and TISL5 (S-c) is Si/Si according to their respective sterility locus. Using SSR molecular marker to detect the segregation of the allele Si and Sj in pollen calli population induced from different hybrid F1, which have different pollen sterility locus, showed that the segregation of allele Si and Sj was distorted. The distorted direction of pollen calli population in vitro was not the same as F2 population in vivo. The quantities of pollen callus carrying Sj were much more than that of carrying Si at S-a and S-c locus, the ratio of Si and Sj were 1:4.81 and 1:1.96 respectively. But the opposite tendency was observed at S-b locus, the ratio of Si and Sj being 1:0.35. At the same time, all these results were undisturbed by either culture medium or culture period.关键词
rice (Oryza sativa L.)/pollen sterility/pollen callus/distorted segregation/SSR markerKey words
rice (Oryza sativa L.)/pollen sterility/pollen callus/distorted segregation/SSR marker分类
农业科技引用本文复制引用
YAO Yan,LU Yong-gen,LIU Xiang-dong,FENG Jiu-huan,ZHANG Gui-quan..Detection of Distorted Segregation in Genotype of Pollen Calli Derived from Hybrid F1 of Cultivated Rice (Oryza sativa L.) Using SSR Markers[J].中国农业科学（英文版）,2006,5(6):412-416,5.基金项目
This work was supported by the National Natural Science Foundation of China (39970048), Guangdong Provincial Natural Science Foundation (990707) and the Fok Ying Tung Education Foundation (71021). （39970048）
