中国实验动物学杂志2001，Vol.11Issue(1)：41-43,3.
鼠痘病毒抗体制备方法的研究
Study on the Preparation Method of Antibody against Ectromelia Virus
巩薇 1卫礼 1贺争鸣1
作者信息
- 1. 中国药品生物制品检定所，北京 100050
- 折叠
摘要
Abstract
Objective To develop a method of production of t he polyclonalantibody and its preservation.Methods 10 KM mice were immunized intraperitoneally four times with an emulsion of Ectromelia virus in complete Freund's adjuvant，together with a single intraperitoneal injection of paraffin li quid.Results Up to 26 ml ascites could be o btained from a single mouse，the mean value of each mouse was 10.6 ml.The total value of ascites produced was 15 times greater tha n that of serum.The antibody titres in the ascites of different mice had some d ifference，from 1∶320 to 1∶5120.With inactivation at 56℃ for 30mins，the an tibody titres had no change in 6 months.In contract，the antibody titre of asci tes showed significant reduction without inactivation.Conclus ion Specific antibody with large quantity and high titre can b e obtained with this method.关键词
腹水/抗体,病毒分类
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巩薇,卫礼,贺争鸣..鼠痘病毒抗体制备方法的研究[J].中国实验动物学杂志,2001,11(1):41-43,3.
