中国药科大学学报2003，Vol.34Issue(1)：41-45,5.
应用放射性免疫方法建立抗早老性痴呆药的M1受体筛选模型
Establishment of M1 Receptor Screening Model for Drugs for Alzheimer's Disease Using Radioimmunoassay Method
摘要
Abstract
AIM:To establish a screening model based on muscarinic receptor type 1(M1) for drugs against Alzheimer's disease (AD). METHOD: Human M1 receptor was expressed in HEK293 (human embryonic kidney) cells, and its activation was measured by the intracellular cAMP (cyclic AMP) level. Exogeneous 3H-cAMP was used to compete the intracellular cAMP binding sites. Acetylcholine chloride was used as a positive drug to ensure the sensitivity of this model. RESULT: HEK293 cell expressing system of human M1 receptor was established. Different concentrations of acetylcholine chloride (10-9～10-4 mol/L) activation of M1 receptor leads to an increase of intracellular cAMP 10.343×10-4～33.754×10-4 pmol/μl. CONCLUTION:This screening model has positive response to M1 receptor agonist and can be used for drug screening.关键词
M1受体/早老性痴呆/cAMP/药物筛选Key words
M1 receptor/Alzheimer's disease/cAMP/drug screening分类
医药卫生引用本文复制引用
张陆勇,江振洲,蔡朝晖,赵小辰,严明,李欣燕..应用放射性免疫方法建立抗早老性痴呆药的M1受体筛选模型[J].中国药科大学学报,2003,34(1):41-45,5.基金项目
This project was supported by National 863 Program Foundation of China(No.2001AA234011) （No.2001AA234011）
