首页|期刊导航|中国农业科学（英文版）|G418-Resistance as a Dominant Selectable Marker for Heterogenous Gene Expression in Antagonist Pichia membranefaciens
中国农业科学（英文版）2005，Vol.4Issue(1)：41-46,6.
G418-Resistance as a Dominant Selectable Marker for Heterogenous Gene Expression in Antagonist Pichia membranefaciens
G418-Resistance as a Dominant Selectable Marker for Heterogenous Gene Expression in Antagonist Pichia membranefaciens
摘要
Abstract
Pichia membranefaciens, which was isolated from the surface of peach fruits, showed effective biocontrol capability against rhizopus rot of peach fruits. Aminoglycoside antibiotic G418 can inhibit the growth of P. membranefaciens. The minimal inhibitory concentration of G418 to P. membranefaciens in YPD medium was 100 μg mL-1. We constructed a phosphoglycerate kinase (PGK) promoter-driven neoR expression cassette, which was called pFL61-neo. The biocontrol yeast P. membranefaciens was transformed with pFL61-neo by lithium acetate method. Expression vector pFL61-neo conferred P. membranefaciens drug resistance to 100 μg mL-1 G418. The transformant could keep a high percentage of plasmid-containing of transformant with 67.87% after 50 generations in non-selective medium. The result showed that P.membranefaciens could recognize the promoter and terminator of PGK and direct the expression of heterologous neoR gene. Expression vector pFL61-neo could exist stably in P. membranefaciens. Therefore, it is feasible to utilize G418-resistance as a dominant selectable marker for heterogenous gene expression in antagonist P. membranefaciens.关键词
Antagonistic yeast, Biocontrol, G418-resistance, Dominant selectable markerKey words
Antagonistic yeast, Biocontrol, G418-resistance, Dominant selectable marker引用本文复制引用
WAN Ya-kun ,TIAN Shi-ping..G418-Resistance as a Dominant Selectable Marker for Heterogenous Gene Expression in Antagonist Pichia membranefaciens[J].中国农业科学（英文版）,2005,4(1):41-46,6.基金项目
This study was supported by the National Science Fund for Distinguished Young Scholars of China (30225030) and the National Natural Science Foundation of China (30430480). （30225030）
