中国药理学与毒理学杂志2004，Vol.18Issue(6)：415-420,6.
木瓜苷对环磷酰胺增强的小鼠接触性超敏反应的影响
Effects of Chaenomeles speciosa glucosides on cyclophosphamide potentiated contact hypersensitivity in mice
摘要
Abstract
AIM To investigate the roles of thymus T lymphocytes subsets in contact hypersensitivity (CHS) and effects of glucosides of Chaenomeles speciosa (GCS) on this response. METHODS CHS model induced by 2,4-dinitro-I-dinitrofluorobenzene (DNFB) and cyclophosphamide (Cy) potentiated CHS model were used. GCS(60,120 and 240 mg·kg-1) were given intragastrically (ig) once daily for 12 consecutive days. Concanavalin A (Con A)-induced lymphocytes proliferation was observed by MTT assay. CD4/CD8 T lymphocytes subsets were measured by flow cytometry and the levels of Con A-induced cytokines from thymocytes were measured by enzyme-linked immunosorbent asssay (ELISA). RESULTS Splenocyte proliferation stimulated by Con A was augmented and CD4+CD8+ T lymphocytes were increased in thymus of mice with CHS. The balance of CD4+Th subsets was changed to favor Th1 and Th3 as shown by the increased interleukin-2 (IL-2) and transforming growth factor β1 (TGF-β1) production and the decreased interleukin-4 (IL-4) production. GCS, similar as the control drug actarit (4-acetylaminophenylacetic acid), could elevate the percentage of CD4+CD8- T lymphocytes and CD4-CD8- T lymphocytes, and reduce the percentage of CD4+CD8+ T cells in Cy-potentiated CHS mice. GCS inhibited the production of IL-2 and TGF-β1, and increased the IL-4 level in cultures of thymocytes from mice with Cy-potentiated CHS. CONCLUSIONGCS inhibits Cy-potentiated mice CHS response and modulates the balance of CD4/CD8 or helper T cells subsets.关键词
木瓜苷/接触性超敏反应/CD4+-T淋巴细胞/CD8+-T淋巴细胞/白细胞介素-2/白细胞介素-4/转化生长因子βKey words
glucosides/Chaenomeles speciosa/contact hypersensitivity/CD4+-positive T lymphocytes/CD8+-positive T lymphocytes/interleukin-2/interleukin-4/transforming growth factor beta分类
医药卫生引用本文复制引用
郑咏秋,魏伟,汪倪萍..木瓜苷对环磷酰胺增强的小鼠接触性超敏反应的影响[J].中国药理学与毒理学杂志,2004,18(6):415-420,6.基金项目
安徽省自然科学基金资助项目(00044411) （00044411）
