中国妇幼健康研究2009，Vol.20Issue(4)：417-419,3.DOI:10.3969/j.issn.1673-5293.2009.04.018
多西他赛联合野生型p53对Hela细胞的抑制作用
A study on inhibition of docetaxel combined with wild type p53 gene on proliferation of Hela cells
宋巧丽 1章根琴 2张军琴1
作者信息
- 1. 河北省张家口教育学院临床系,河北,张家口,075000
- 2. 浙江省金华市人民医院妇产科,浙江,金华,321000
- 折叠
摘要
Abstract
Objective To investigate inhibitory effects of docetaxel alone and docetaxel combined with wild type p53(wt p53) on proliferation of human cervical cancer cells-Hela cells. Methods The Hela cells were divided into two groups: wt p53 transfected Hela cells group(p53-Hela group, obtained by using lipofection-mediated gene transfection method) and blank control group (not transfected with wt p53). The expression of wt p53 was deteced by using reverse transcription polymerase chain reaction(RT-PCR) method. Docetaxel acted on Hela cells and p53-Hela cells. After 24 hours and 48 hours,the morphology of the cells in the two groups were observed respectively and OD450 were tested by using cell counting kit-8(CCK-8) to caculate the inhibition rate. Results After transfection with wt p53, the expression of p53mRNA in p53-Hela group was increased, and there was a significant difference as compared with that in Hela group (t=-17.504, P<0.01). Docetaxel exerted an inhibitary effect on the Hela cell line in the two groups at all action times (Fp53-Hela=53.500,P<0.01;FHela=430.225,P<0.01). This cellular inhibitory effects showed dose and time dependent manners. Conclusion Docetaxel alone exerts an inhibitory effect on Hela cells and this inhibitary effect could be increased when docetaxel is combined with wt p53.关键词
宫颈肿瘤/Hela细胞/野生型p53基因/多西他赛Key words
cervical neoplasm/Hela cell/wild type p53 gene (wt p53)/docetaxel分类
医药卫生引用本文复制引用
宋巧丽,章根琴,张军琴..多西他赛联合野生型p53对Hela细胞的抑制作用[J].中国妇幼健康研究,2009,20(4):417-419,3.
