摘要
Abstract
BACKGROUND: Artificial nerve grafts of human hair keratin are a kind of biological products. It has low antigenicity, absorbability and stimulation to nerve fiber growth following specific biochemistry. It is hoped to have better effect than other artificial nerve grafts.
OBJECTIVE: To investigate the anatomy and histocompatibility of artificial nerve grafts of the human-hair keratin, and to observe its effects on the repair of peripheral nerves.
DESIGN, TIME AND SETTING: Randomized, controlled, animal experiment. The study was performed at the Animal Central Laboratory of Affiliated Hospital of Qingdao University Medical College between November 2006 and June 2008.
MATERIALS: Artificial nerve grafts of human hair keratin is a compound of human hair processed by specific controlled biochemistry based on ground substance, embedded with a layer of biological membrane. It has low antigenicity, absorbability and stimulation to nerve fiber growth following specific biochemistry.
METHODS: Eighteen Wistar rats were randomly divided into three groups. The sciatic nerve, 10 mm, was removed and transplanted with human-hair keratin graft, skeletal muscle and untreated hair, respectively.
MAIN OUTCOME MEASURES: The characteristics of histomorphology and anatomy were observed at 8, 12, 24 weeks after the surgery.
RESULTS: White tissues appeared between the broken ends of the sciatic nerve at 8 post-operative week in the graft group, and appeared in the graft space in human-hair keratin at the 12th week. At the 24th week, a large amount of infantile myelinated nerve fibers were observed under optical microscope regenerating around the human hair, which was partially degraded and absorbed. Schwann cells were observed under an electron microscope and myelinization.
CONCLUSION: The artificial nerve grafts of the human-hair keratin are well compatible with the body tissues, and could induce nerve regeneration.
关键词
人发角蛋白/非神经移植材料/周围神经/组织相容性分类
医药卫生
