首页|期刊导航|高技术通讯（英文版）|Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341
高技术通讯（英文版）2005，Vol.11Issue(4)：421-426,6.
Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341
Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341
摘要
Abstract
The complete [NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in between 98bp. There is a stem-loop structure, downstream of the hoxY gene, serving as a transcription terminator. The deduced amino acid sequences of HoxY and HoxH consist of acidic amino acid of 15.8% and 12.0%; alkalitropic amino acid of 11.9% and 15.3%; hydrophobic amino acid of 46.3% and 41.0%, respectively. The similarities of hoxY and hoxH genes in Arthrospira platensis FACHB341 to their homologues in other cyanobacteria were compared respectively. The secondary structures and 3D models of small and large subunits of [NiFe]-hydrogenase were predicted by using 3D-PSSM.关键词
Arthrospira platensis, [NiFe]-Hydrogenase, in-vitro cloning, sequence analysis, secondary structure, 3D modelKey words
Arthrospira platensis, [NiFe]-Hydrogenase, in-vitro cloning, sequence analysis, secondary structure, 3D model分类
生物科学引用本文复制引用
Zhang Xiaohui,Sui Zhenghong,Yoshihiro Shiraiwa,Koji Iwamoto,Zhang Xuecheng..Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341[J].高技术通讯（英文版）,2005,11(4):421-426,6.基金项目
Supported by Cooperation Project of Sino-Japan Core Universities. （）
