中华医学杂志（英文版）2001，Vol.114Issue(4)：422-423,2.
Genetic analysis of wild-type hepatitis A virus strains
Genetic analysis of wild-type hepatitis A virus strains
陈勇 1毛江森 1洪艳 1杨连华 1凌志强 1俞为群1
作者信息
摘要
Abstract
Objective To clarify the distribution of hepatitis A virus (HAV)genotype in geographical regions of China. Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions. Viral RNA was recovered from stool or serum by proteinase K digestion and phenol-chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT-PCR) amplification. The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique. Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ. About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5.3% variability. Sequence homology between genotype ⅠA and ⅠB varied from 88.7% to 92.3%. Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB. Epidemiologically related strains may be identical or closely related in sequence.关键词
基因分型/甲型肝炎病毒/基因区Key words
genetic analysis/hepatitis A virus/geographical region甲型肝炎病毒野毒株基因分型的研究陈勇 毛江森 洪艳 杨连华 凌志强 俞为群分类
医药卫生引用本文复制引用
陈勇,毛江森,洪艳,杨连华,凌志强,俞为群..Genetic analysis of wild-type hepatitis A virus strains[J].中华医学杂志（英文版）,2001,114(4):422-423,2.
