植物研究2005，Vol.25Issue(4)：423-426,4.
烟草叶片愈伤组织BECTLIN Ⅰ基因的表达
Expression of BECTLIN Ⅰ gene in the leaf callus of Nicotiana benthamiana
摘要
Abstract
We induced the callus from the leaves of Nicotiana benthamiana cultured in the MS medium and grew in the green house. Then we used the callus to detect the expression of BECTLIN Ⅰ gene which was correlated with Programmed Cell Death (PCD). The detection of RT-PCR showed that the fragment of BECTLIN Ⅰ gene cDNA was amplified in the leaves of the in-vitro plantlets subcultured in the medium and those of the plotted plant seedling, but BECTLIN Ⅰ gene expressed continually and steadily in the leaves callus of the plantlets. This probably indicated that auxins in the medium had some effect on the expression of BECTLIN Ⅰ gene. At the same time, PCD might occur in the course of the formation of the leaves callus, and in the state of dedifferentiation there probably appeared the embryoblasts with the same configuration and function.关键词
烟草/愈伤组织/BECTLIN Ⅰ基因/RT-PCR/细胞程序化死亡Key words
Nicotiana benthamiana/callus/BECTLIN Ⅰ gene/RT-PCR/programmed cell death分类
农业科技引用本文复制引用
祖元刚,刘英,郭晓瑞,孟庆焕,祁长青,姜洋..烟草叶片愈伤组织BECTLIN Ⅰ基因的表达[J].植物研究,2005,25(4):423-426,4.基金项目
Supported by the State Key Basic Research Project (G19990160) and the Key Project of Chinese Ministry of Education( 104191 ) （G19990160）
