昆明医学院学报2001，Vol.22Issue(1)：42-44,3.
细菌质粒提取方法的研究
The Study on Improving Method of Extracting Bacteria Plasmid
摘要
Abstract
Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity． Result The eight strip s of Ecliv 5l7 can not be found completely in original method, but can be found in our one．We found the largest plasmid molecular weight was l86.3?kb, and t he minimum was l.l?kb from each 30 strains STA, EC, PA． Plasmid profile is alike through repeating ex p eriment three times． Conclusion:The method can be widely used in extracting bac teria plasmid and fit gram-negative and gram-positive bacteria whenever molecular weight ma x or min． These conditions can be obtained in general laboratory．关键词
质粒/革兰氏阴性菌/革兰氏阳性菌/分子量分类
医药卫生引用本文复制引用
单斌,陈端,骈淮燕,荀云珍..细菌质粒提取方法的研究[J].昆明医学院学报,2001,22(1):42-44,3.
