分析化学2001，Vol.29Issue(1)：42-44,3.
吖啶橙-罗丹明6G能量转移荧光法测定痕量磷
The Energy Transfer Fluorescence Method for Determination of Trace Phosphorous with Acridine Orange-Rhodamine 6G
刘保生 1王桂华 1孙汉文 1鲍所言 1黄永章1
作者信息
摘要
Abstract
It was found that at the condition of λex/ λem=450／556 nm，in thedodecyl benzene sodium sulfonate solution, the effective energy transfer could occur between AO and R6G. The fluorescence intensity of R6G was diminished by molybdophosphate which was formed by the reaction between orthophosphate and molybdate in hydrochloric acid medium. The detection limit of this method was 5 ng/L. The range of determination for phosphorous was 0.05～0．70 μg/L. The relative standard deviation for the determination of 0.5 μg/L phosphorous was 6% (n=13). The recoveries for the addition of 0.2～0．4 μg/L phosphorous were 95%～110％. The method has been applied to the determination of phosphorous in water samples and soil samples with satisfactory reuslts.关键词
磷,吖啶橙,罗丹明6G,能量转移,荧光分类
化学化工引用本文复制引用
刘保生,王桂华,孙汉文,鲍所言,黄永章..吖啶橙-罗丹明6G能量转移荧光法测定痕量磷[J].分析化学,2001,29(1):42-44,3.
