中国人兽共患病杂志2000，Vol.16Issue(6)：42-44,3.
弓形虫DNA疫苗免疫鼠体内重组质粒分布的探讨
STUDY ON DISTRIBUTION OF RECOMBINANT PLASMID IN MICE
IMMUNIZED BY DNA VACCINE OF TOXOPLASMA GONDII
占国清 1李国光 1吴少庭 2郑春福 3林敏2
作者信息
- 1. 同济医科大学流行病学教研室
- 2. 深圳市卫生防疫站深圳，518020
- 3. 重庆医科大学寄生虫学教研室
- 折叠
摘要
Abstract
Aim Directly immunize BALB/c mice via intramuscular injection with DNA vaccines pBK-P30、pBK-P22, to observe the distribution of the recombinant plasmids in different organs and tissues of mice. Methods Recombinant plasmids pBK-P30、pBK-P22 were injected into the BALB/c mice via muscles ,after 5 weeks immunization,taking lung ,heart liver,spleen,muscle and blood,extracting tissue DNA as templates,amplifying P30 and P22 gene fragments by means of polymerase chain reaction (PCR)and identifying the amplification products by agarose gel electrophoresis. Results P30 gene fragments were amplified out from those tissues of thepBK-P30 vaccinated group mice, P30 and P22 gene framents were amplified out from those tissues of the pBK-P30&pBK-P22 vaccinated group mice respectively,the amplified fragments size of P30 and P22 were 1027bp and 593bp respectively,which were in accordance with the expected ones. NS and pBK-CMV control group mice could not be amplified any fragment. But those specific gene fragments were only amplified specially from blood after 10 weeks immunization. Conclusion After 10 weeks immunization, the combinant plasmids distribute different organs and tissues of the BALB/c mice immunized by DNA vaccines of Toxoplasma gondii ,but only blood was Detected out the recombinant plasmids.关键词
弓形虫/DNA疫苗/分布Key words
Toxoplasma gondii/DNA vaccine/Distribution分类
医药卫生引用本文复制引用
占国清,李国光,吴少庭,郑春福,林敏..弓形虫DNA疫苗免疫鼠体内重组质粒分布的探讨[J].中国人兽共患病杂志,2000,16(6):42-44,3.
