癌变·畸变·突变2008，Vol.20Issue(6)：424-428,5.
Hes 1基因诱导小鼠肝原始细胞分化为胆管上皮细胞
Hes 1 Induced Differentiation of Mouse Liver Progenitor Cells to Cholangiocytes
摘要
Abstract
BACKGROUND AND AIM:To study the differentiation of mouse liver epithelial progenitor cells(LEPCs)to cholangiocytes induced by hairy and enhancer of split 1(Hes1)gene in vitro.MATERIALS AND METHODS:The Hes1 gene was amplified by PCR from mouse tail genomic DNA and subcloned into pEGFP-C1 and pcDNA3.1 vector.LEPCs were tranfected and performed in vitro.Cholangiocyte markers,CK19,GGT,HNF6 and HNF1B;hepatocyte markers,GS and BGP;hepatic oval cell marker,Thy-1 were assayed with RT-PCR and Real-time PCR.The fluorescence of the LEPCs tagged with EGFP was observed in vitro.RESULTS:Cholangiocyte markers,CK19,GGT,HNF6 and HNF1B, were up-regulated.Hepatocyte markers GS and BGP,and hepatic oval cell marker Thy-1,were down-regulated. CONCLUSION:It indicated that LEPCs differentiated to cholangiocytes initially in vitro.and Hesl might play important mles in the differentiation of LEPCs and influence cholangiocyte versus hepatocyte fate determination.关键词
Hes1/肝原始细胞/分化/胆管上皮细胞Key words
Hes1/liver epithelial progenitor cell/differentiation/cholangiocyte分类
生物科学引用本文复制引用
田棣,李文林,攸璞,金彩霞,朱吉,张南,胡以平..Hes 1基因诱导小鼠肝原始细胞分化为胆管上皮细胞[J].癌变·畸变·突变,2008,20(6):424-428,5.基金项目
原国家自然基金项目(30470876,30600326,30472141) （30470876,30600326,30472141）
国家863计划项目(2006AA02Z474) （2006AA02Z474）
上海重点基础科学计划研究项目(03DJ14020,06W14001) （03DJ14020,06W14001）
