中德临床肿瘤学杂志(英文版)2002，Vol.1Issue(1)：42-47,6.
CCK、Gastrin对胆管癌细胞凋亡的调控
The Regulating Effect of CCK and Gastrin on Apoptosis of Bile Duct Carcinoma Cells
摘要
Abstract
Objective Probe into the influence and the mechanisms of CCK and gastrin on the apoptosis of bile duct carcinoma cells.Methods By taking beauvericin as the revulsant to the apoptosis of bile duct carcinoma cells, the influence of CCK and gastrin on theapoptosis of bile duct carcinoma cells was investigated by using the techniques such as TUNEL fluorescent staining, stream mode cell de-tecting instrument and reverse bcl-2 oligonucleotide. Techniques of immunohistochemistry, in situ hybridization, flow cytometry (FCM),RT-PCR were used to study the roles of apoptosis-related genes bcl-2 and baxResults After beauvericin 40μM worked for 12 h, the survival rate of QBC939 bile duct carcinoma cells was decreased by 35% - 40%.About 80% of the bile duct carcinoma cells showed various degrees of apoptosis. CCK and gastrin could upregulate the threshold value ofthe apoptosis of bile duct carcinoma cells, which could be inhibited by L60, L18 and reverse bcl-2 oligonucleotide. In terms of both tran-scription and translating levels, CCK and gastrin could obviously promote the genetic expression of bc1-2, but had no influence on the ge-netic expression of bax. Addition of CCK-A receptor or CCK-B/gastr in receptor antagonist could remarkably inhibit the expression of bc1-2 boosted by gastrin-17 and CCK-8S.Conclusion CCK and gastrin inhibited the apoptosis of bile duct carcinoma cells through upregulating the genetic expression of bcl-2.Theoretically, this research has expanded our understanding to the mechanism of CCK and gastrin in controlling the growth of tumors, en-riched our view to the mechanism of apoptosis of alimentary tract tumors, and has provided a new thinking for the assistant treatment to bileduct carcinoma cells as well.关键词
胆囊收缩素/胃泌素/凋亡/胆管癌/bcl-2/baxKey words
cholecystokinin/gastrin/apoptosis/bile duct carcinoma/bcl-2/bax分类
医药卫生引用本文复制引用
马宽生,张丰深,何振平,董家鸿..CCK、Gastrin对胆管癌细胞凋亡的调控[J].中德临床肿瘤学杂志(英文版),2002,1(1):42-47,6.基金项目
Supported by National Natural Science Foundation of China. Project number:39670711 （）
