林业科学2008，Vol.44Issue(10)：42-48,7.
7个砂梨品种S基因型的确定及1个新S-RNase基因的分离鉴定
Determination of S-Genotypes of Seven Cultivars and Identification of a Novel S-RNase Allele in Pyrus pyrifolia
摘要
Abstract
Pyrus pyrifolia is a commercially important fruit tree which exhibits gametophytic self-incompatibility (GSI). It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, seven cultivars of P. pyrifolia were used for S-genotype analysis by PCR-based molecular method with primers designed from conserved sequences of known pear S-RNases. Amplified products were analyzed by 1.8% agarose gel electrophoresis. In each of the four cultivars ' Chubixiang', ' Huangpishui', ' Zhenghedaxueli' and ' Hongtaiyang', two expected bands were generated. The amplified products in the other three cultivars did not show length polymorphism, and therefore were further separated by 6% polyacrylamide gel electrophoresis (PAGE). A total of 14 purified fragments from the seven cultivars were cloned and sequenced. Sequence analysis revealed that 10 alleles with typical structural features of pear S-RNase were identified, of which one from 'Zhenghedaxueli', with 494 bp, was determined as a new S-RNase allele that was tentatively denominated as S43-RNase (GenBank accession No. EF566873). RT-PCR revealed that the S43-RNase was expressed specifically in the styles, which is consistent with the expression pattern of S-RNases. Comparison of genomic and cDNA sequences revealed there was an intron of 294 bp in the S43-RNase gene. The deduced amino acid sequence shared 65 % to 92% similarity with other Maloideae S-RNases. This study will be helpful in pear production and breeding programs.关键词
砂梨/配子体自交不亲和性/PCR/自交不亲和基因型/S-RNaseKey words
Pyrus pyrifolia/gametophytic self-incompatibility (GSI)/PCR/S-genotype/S-RNase分类
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张琳,谭晓风,周建,何小勇,袁德义,胡姣,龙洪旭..7个砂梨品种S基因型的确定及1个新S-RNase基因的分离鉴定[J].林业科学,2008,44(10):42-48,7.基金项目
Foundation project: Supported by the Key Project of State Forestry Administration (2006 - 12) and Scientific Research Fund of Central South University of Forestry & Technology (2008007B). （2006 - 12）
