中华医学杂志（英文版）2003，Vol.116Issue(3)：428-431,4.
Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells
Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells
万虹 1安沂华 1张泽舜 1张亚卓 1王忠诚1
作者信息
- 1. Beijing Neurosurgical Institute, Beijing 100050, China
- 折叠
摘要
Abstract
Objective To explore the factors which induce differentiation of embryonic neural stem cells. Methods Rat embryonic neural stem cells were co-cultured with newborn rat Schwann cells in serum-free medium. The phenotype and specific-markers including tubulin-β, glial fibrillary acidic protein (GFAP) and galactorcerebroside (GalC), were domonstrated by phase contrast microscopy and double immunofluorescence staining. Results Overall, 80%±5% of neural stem cells protruded several elongated processes and expressed tubulin-β antigen at high levels, while 20±3% of them protruded several short processes and were GalC or GFAP positive. Conclusion The factors secreted by Schwann cells could induce rat embryonic neural stem cell to differentiate.关键词
Schwann cells/stem cells/cell differentiationKey words
Schwann cells/stem cells/cell differentiation分类
医药卫生引用本文复制引用
万虹,安沂华,张泽舜,张亚卓,王忠诚..Differentiation of rat embryonic neural stem cells promoted by co-cultured Schwann cells[J].中华医学杂志（英文版）,2003,116(3):428-431,4.
