昆虫学报2004，Vol.47Issue(4)：429-433,5.
cAMP和cGMP对棉铃虫神经细胞高电压激活钙通道的调节作用
Modulations by cAMP and cGMP of the high-voltage activated Ca2+ channels in the central neurons of the cotton bollworm, Helicoverpa armigera
摘要
Abstract
The effects of cAMP and cGMP on the high-voltage activated (HVA) Ca2+ channels in the central neurons isolated from the 3rd instar larvae of the cotton bollworm (Helicoverpa armigera ) were studied using the whole-cell patch clamp technique. With barium as current carrier, the currents of Ca2+ channels were recorded by blocking Na+ and K+ currents.Extracellular application of 0. 1 mmol/L forskolin, a stimulator of adenylyl cyclase (AC), had no effect on the peak amplitude of Ba2+ currents. In contrast, 1 mmol/L cGMP in the intracellular solution suppressed the current amplitude significantly and the inhibition was time- and dose-dependent. The results suggest that the activities of HVA Ca2 + channels in the cotton bollworm may not be affected by the elevated cAMP, but can be inhibited by the increased cGMP.关键词
棉铃虫/环磷酸腺苷/环磷酸鸟苷/高电压激活钙通道/膜片钳Key words
Helicoverpa armigera/cyclic AMP/cyclic GMP/high-voltage activated calcium channel/patch clamp分类
生物科学引用本文复制引用
李杰,杜育哲,佟帆,贺秉军,刘安西..cAMP和cGMP对棉铃虫神经细胞高电压激活钙通道的调节作用[J].昆虫学报,2004,47(4):429-433,5.基金项目
国家自然科学基金项目(30270884,30100119) （30270884,30100119）
