外科理论与实践2009，Vol.14Issue(4)：430-434,5.
不同结构的中/长链脂肪乳对肿瘤病人免疫细胞的影响
Impact of different structure medium-and long-chain triglyceride emulsions on immune cells in gastrointestinal tumor patients
施咏梅 1陈雪华 2曹伟新1
作者信息
- 1. 上海交通大学医学院附属瑞金医院,临床营养科,上海,200025
- 2. 上海交通大学医学院附属瑞金医院,上海消化外科研究所,上海,200025
- 折叠
摘要
Abstract
Objective To study the influence of different structure medium-and long-chain triglyceride emulsions on immune cells functions in gastrointestinal tumor patients. Methods ①Human whole blood cells were cultured in vitro with the structured triglyceride (STG) emulsion or physical mixture of medium-and long-chain triglyceride (MCT/LCT) emulsion with a final concentration of 10 mg/mL, 20 mg/mL and 100 mg/mL respectively. Taking the FITC-dextran particle as a marker of phagocytosis, the percentage of neutrophil phagocytosis was detected by flow cytometry after 2 hours of the incubation. ② The isolated lymphocytes were incubated in the STG emulsion or MCT/LCT emulsion with a final concentration of 10 mg/mL, 20 mg/mL and 100 mg/mL respectively. After 24 hours of incubation, the apoptosis rate of lymphocytes was measured by Annexin V/PI staining with flow cytometry. Resujts ①With lipid emulsions at the concentration of 10 mg/mL there was no difference in neutrophil phagocytosis rate among the STG, MCT/LCT and the blank groups. ②At the concentration of 20 mg/mL, the neutrophil phagocytosis rate in the STG group was significant lower than that in the MCT/LCT group(P<0.05), but there was no significant difference between the STG and the blank groups.③ At the concentration of 100 mg/mL, the neutrophil phagocytosis rate in the STG group was significant lower than that in the MCT/LCT and blank groups (P<0.05). ④The tendency of neutrophil phagocytosis decreased following the up-regulation of lipid emulsion dosage (P<0.05). ⑤After 24-hour cell culture, there was no obvious change in the apoptosis rate of lymphocytes with the changes in concentration of lipid emulsions in the STG or MCT/LCT groups. Conclusions There is no significant change in neutrophil phagocytosis function and lymphocyte apoptosis with structured triglyceride emulsion at a regular dosage. However, neutrophil phagocytosis function is inhibited with structured triglyceride emulsion at a high dosage. No such phenomena is observed when using the MCT/LCT emulsion.关键词
脂肪乳/中性粒细胞/淋巴细胞/吞噬/凋亡Key words
Lipid emulsion/ Neutrophil/ Lymphocyte/ Phagocytosis/ Apoptosis分类
医药卫生引用本文复制引用
施咏梅,陈雪华,曹伟新..不同结构的中/长链脂肪乳对肿瘤病人免疫细胞的影响[J].外科理论与实践,2009,14(4):430-434,5.
