首页|期刊导航|中华医学杂志（英文版）|Anti-CD25 monoclonal antibody modulates cytokine expression and prolongs allografts survival in rats cardiac transplantation
中华医学杂志（英文版）2003，Vol.116Issue(3)：432-435,4.
Anti-CD25 monoclonal antibody modulates cytokine expression and prolongs allografts survival in rats cardiac transplantation
Anti-CD25 monoclonal antibody modulates cytokine expression and prolongs allografts survival in rats cardiac transplantation
摘要
Abstract
Objective To investigate the role of anti- interleukin-2 receptor(CD25) monoclonal antibody in the regulation of cytokine mRNA expression of IL-1β, IL-2, CD25, IL-4, IL-5, IL-6, IL-10, tumour necrosis factor-α (TNFα), and interferon-γ (IFNγ) in cardiac allografts to elucidate its immunological mechanism and role in rats that have undergone cardiac transplantation. Methods These in vivo studies were conducted using a rat MHC mismatch SD to Wistar heterotopic cardiac transplant model. Simulect, an anti-CD25 antibody, was used to prevent allograft rejection. An increase in the rate of allograft survival was observed. Rats were sacrificed on day 1, 3, 5, 7, 9, 11, 14 post-transplantation and hearts were harvested for furgher study. Cytokine mRNA expression was determined by semiquantitative RT-PCR. Results In the control group, cardiac allografts were rejected at 8.3±1.7 days after transplantation (±s). The rats who received CsA rejected the cardiac allograft at 26.4±5.7 days post-transplant. Allograft survival of Simulect-treated rats was 29.2±7.1 days (P<0.05 vs controls). Rats treated with simulect and CsA had the longest survival of 55.0±11.6 days (P<0.001 vs controls). CD25 mRNA expression in the heart tissue samples of treated rats was undetectable or very weak. However, the untreated group, CD25 expression increased, although anti-CD25 decreased this CD25 expression in the heart graft. Furthermore, in untreated allografts, IL-2, TNFα and IFN-γ were strongly expressed, an effect that markedly decreased after simulect treatment. Finally, IL-4, IL-5, IL-6 and IL-10 expression was strong in anti-CD25-treated allografts. Conclusions These results suggest that anti-CD25 antibody treatment may not only neutralize CD25 activity but also play a role in altering cytokine mRNA expression and prolong the survival of allografts.关键词
transplantation/allograft rejection/simulect/cytokineKey words
transplantation/allograft rejection/simulect/cytokine分类
医药卫生引用本文复制引用
夏家红,蒋雄刚,黄毅,张凯伦,肖诗亮,杨辰垣..Anti-CD25 monoclonal antibody modulates cytokine expression and prolongs allografts survival in rats cardiac transplantation[J].中华医学杂志（英文版）,2003,116(3):432-435,4.基金项目
This work was supported by a grant from the National Natural Science Foundation of China (No. 39670720). （No. 39670720）
