中国临床康复2003，Vol.7Issue(32)：4325-4327,3.
构建重组肿瘤基因疫苗对肿瘤进展及生存率影响的实验结局
Effect of recombinant genetic vaccine against tumors on tumor progression and survival rate of mice
摘要
Abstract
AIM:To study the immunological effect of genetic vaccine co- administered with murine IL- 12 against mouse mastocytoma P815. METHODS:Antigen- specific P1A gene of P815 mastocytoma was transduced into the eukaryotic expression vector pCI- neo to prepare the genetic vaccine.Mouse models of P815 tumor were established by subcutaneous injection of P815 cells,and the growth of the tumor, the stimulation of cytotoxic T cells and production of specific antibodies were observed following injection of the recombinant vaccine into mice muscle alone or together with IL- 12- expression plasmids. RESULTS:The recombinant vaccine could be expressed in vitro, with specific release rate of cytotoxic T cells reaching 40% after injection alone, which mounted up to 60% when IL- 12- expressing plasmid was co- administered.About 30% of the recombinant P1A vaccine- inoculated mice showed signs of tumor regression, and when IL- 12- expressing plasmid was used,the regression could reached 50% .No antibodies, however, could be detected in the sera of any of the immunized mice. CONCLUSION:Tumor- specific immunological response can be elicited by recombinant P1A vaccine through cellular immunity,and IL- 12 can enhance the immune effect of this vaccine.关键词
疫苗,合成/肿瘤/基因/模型,生物学/小鼠分类
医药卫生引用本文复制引用
倪兵,吴玉章,李艳秋,唐艳,邹丽云,周伟,贾正才,林治华..构建重组肿瘤基因疫苗对肿瘤进展及生存率影响的实验结局[J].中国临床康复,2003,7(32):4325-4327,3.基金项目
973国家计划资助项目(2001CB510001) （2001CB510001）
