青岛大学医学院学报2009，Vol.45Issue(5)：433-436,4.DOI:10.3969/j.issn.1672-4488.2009.05.010
应用小干扰RNA技术阻断VEGF-C基因体内抑制乳癌细胞增殖
BLOCKING VASCULAR ENDOTHELIAL GROWTH FACTOR-C GENE TO INHIBIT BREAST CANCER CELL PROLIFERATION IN VIVO BY USING siRNA TECHNIQUE
摘要
Abstract
Objective Using chemically-modified siRNA technique, vascular endothelial growth factor-C (VEGF-C) gene in transplanted breast cancer in nude mice was blocked and its effects on the growth of transplanted tumor investigated. MethodsHuman breast cancer cells MCF-7 were subcutaneously transplanted into the female mice, those with tumor-positive were rando-mized to three groups as VEGF-C siRNA, liposome and control, with five mice in each group. For VEGF-C siRNA group: VEGF-C siRNA 1 mg/kg and in vivo jetPEITM were injected locally; for liposome group: intra-tumor injection of in vivo jetPEITM and PBS was done; for control group: PBS alone was given. The medication was given every three days for eight times. The experimental animals were sacrificed 22 days later and tumors taken. Semiquantitative RT-PCR was applied for VEGF-C Mrna detection, and Western blotting for VEGF-C. Results The growth of tumor in VEGF-C siRNA group was greatly inhibited, the expressions of VEGF-C gene and protein significantly decreased (F=73.64-197.15,q=8.74-25.56,P<0.05). In control group, no significant changes were noted in terms of various items. Conclusion The RNAi mediated by chemically modified siRNA can efficiently down regulate VEGF-C expression in nude mice with transplanted breast cancer to restrain the growth of the tumor, which is a novel potential gene therapy of this condition.关键词
RNA干扰/乳腺肿瘤/血管内皮生长因子CKey words
RNA interference/Breast neoplasms/Vascular endothelial growth factor C分类
临床医学引用本文复制引用
李鲲,葛银林,张金玉..应用小干扰RNA技术阻断VEGF-C基因体内抑制乳癌细胞增殖[J].青岛大学医学院学报,2009,45(5):433-436,4.基金项目
Qingdao Science and Technology Project (07-2-1-7-nsh) （07-2-1-7-nsh）
