中国病理生理杂志2003，Vol.19Issue(4)：433-437,5.
MNNG对哺乳类细胞JNK/SAPK及p38MAPK作用及其信号源研究
Low concentration MNNG inhibition of JNK/SAPK is independent of a nuclear signal while MNNG activation of p38MAPK may depend on a nuclear signal
摘要
Abstract
AIM: To identify the effect of alkylating agent N-methyl-N'- nitro-N-nitrosoguanidine (MNNG) with low concentration on JNK/SAPK and p38MAPK and the origins of JNK/SAPK and p38MAPK cascade.METHODS: p38 and JNK kinase activity were detected by immunoprecipitation and Western immunoblotting in intact and enucleated Vero cells.RESULTS: With the same experimental conditions, low concentration of MNNG inhibited JNK kinase in both intact cell and enucleated Vero cell. MNNG activated p38 kinase in intact cell while no effect on p38 kinase in enucleated cell was observed.CONCLUSION: Inhibition of JNK/SAPK by low concentration of MNNG was independ of a nuclear signal while MNNG activation of p38MAPK may depend on a nuclear signal.关键词
烷化剂/DNA损伤/诱变/信号转导Key words
Alkylating agents/DNA damage/Mutagenesis/Signal transduction分类
医药卫生引用本文复制引用
竺可青,余应年,章锁江..MNNG对哺乳类细胞JNK/SAPK及p38MAPK作用及其信号源研究[J].中国病理生理杂志,2003,19(4):433-437,5.基金项目
National Natural Science Foundation of China (No.39830210, No.39870684) （No.39830210, No.39870684）
Doctoral Project Foundation of Ministry of Education PR China (1999033557) （1999033557）
Nature Science Foundation of Zhejiang Province (No.29801) （No.29801）
