中国临床康复2003，Vol.7Issue(32)：4340-4341,2.
应用组织工程方法体外培养人牙囊细胞与I型胶原的合成
Culture of dental follicle cells from human molars with tissue engineering and synthesis of I type collagen
摘要
Abstract
AIM:The dental follicle is important for the eruption of teeth.The objective of this study is to culture the human dental follicle cells in vitro and research the characterization of these cells to investigate the effects of dental follicle in the teeth eruption. METHODS: The dental follicles were dissected from the lower third impacted molars (uneruption), and then cultured in a medium. The cultured cells grew to confluence and were through 3 passages before experimentation. They were observed with microscopy and scanning electron microscopy. Immunohistochemical staining technique and immunofluorescence staining showed the expression of vimentin and type Ι collagen respectively in cultured human dental follicle cells and localization of them in cytoplasm. RESULTS: Dental follicle cells cultured were elongate shape, exhibited fibroblastic characteristics, and scanning electron microscopy revealed that there were a lot of granules in the surface of cultured cells.The expression of type Ι collagen and vimentin showed positive; the localization of type Ι collagen and vimentin were in the cytoplasm surrounding of nucleus. CONCLUSION: The results indicate that the human dental follicle cells can be cultured in vitro, exhibit fibroblastic characteristics, derive from intermediate filaments, and synthesize type Ι collagen.关键词
牙囊/显微镜检查/电镜/胶原/免疫组织化学分类
医药卫生引用本文复制引用
金作林,林珠,罗颂椒..应用组织工程方法体外培养人牙囊细胞与I型胶原的合成[J].中国临床康复,2003,7(32):4340-4341,2.基金项目
陕西省自然科学基金(2002C2-14) （2002C2-14）
