中国林学(英文版)2002，Vol.4Issue(2)：43-47,5.
光肩星天牛纤维素酶的性质研究
Characteristics of Cellulases from Anoplophora glabripennis Motsch ( Coleoptera: Cerambycidae)
摘要
Abstract
The property of major cellulases from the guts of Anoplophora glabripennis larvae have been char acterized. The optimal temperatures of both β-l,4-glucosidase (β-glucosidase) and endo-β-1,4-endoglucanase (endoglucanase, Cx) are 40℃. The β-glucosidase was optimally active at pH 4.8, while the optimal activity of the endoglucanase occurred at pH 4.4-5.6. The endoglucanase was active with a wide range of pH and temperature, the levels of activity from 25℃ to 50℃ were more than 80%, and the activity remained 60% between pH 3.2 and pH 7.2. The endoglucanase exhibited higher thermal stability than β-glucosidase. Both enzymes lose their activities by heat treatment at 60℃. Two isozymes of endoglucanase were detected in sodium carboxymethylcellulose polyacrymide gels (CMC-gel) by chemical colorization, and purified by elution from the gel slices. The molecular weights of the two isozymes were estimated as 26kD and 39kD respectively. Moreover molecular characteristics of the two isozymes are currently underway.关键词
光肩星天牛/纤维素酶的特性/内切葡聚糖酶/β-葡萄糖苷酶/同工酶Key words
Anoplophora glabripennis/characteristics of cellulases/endoglucanase/β-glucosidase/isozymes分类
农业科技引用本文复制引用
陈敏,卢孟柱,张志毅..光肩星天牛纤维素酶的性质研究[J].中国林学(英文版),2002,4(2):43-47,5.基金项目
Supported by the National Natural Science Foundation of China (Grant No. 39900116) （Grant No. 39900116）
